Uses And Principle Of Operation Of Biosafety Cabinet

This chapter discusses the purpose principle of operation specifications and applications of biosafety cabinet bsc.

Uses and principle of operation of biosafety cabinet.

Primary containment which is the protection of personnel and the immediate laboratory environment from exposure to infectious agents is provided. A biosafety cabinet bsc also called a biological safety cabinet or microbiological safety cabinet is an enclosed ventilated laboratory workspace for safely working with materials contaminated with or potentially contaminated with pathogens requiring a defined biosafety level. In varying degrees a laminar flow biological safety cabinet is designed to provide three basic types of protection. Improper use of a biological safety cabinet can result in contaminated cultures and expose workers to infectious organisms.

The term containment describes safe methods or properly managing infectious agents in the laboratory environment where they are being handled or maintained. With proper techniques activities within the work area can be performed safely minimizing the chance of cross contamination. Biological safety cabinet a piece of equipment designed to protect the operator the laboratory environment and work materials from exposure to infectious aerosols and splashes that may be generated when manipulating substances containing infectious agents such as viruses bacteria and primary tissue cultures. Improved principles ofbiological safety cabinet design true laminar airflow unidirectional airflow moving along parallel flow lines at a constant velocity minimizes air turbulence within the work zone.

Product protection to avoid contamination of the work experiment or process. Follow these guidelines for working safely in a biological safety cabinet bsc. Personnel protection from harmful agents inside the cabinet. The purpose of containment is to reduce or eliminate the exposure to potentially hazardous agents of laboratory workers other persons and the outside environment.

The terms biological safety cabinet and biosafety cabinet have been widely used to describe a variety of containment devices equipped with hepa filter s designed to provide personnel or both personnel and product protection from biohazardous materials. A bsc serves as a means to protect the laboratory worker and the surrounding e. Use of biological safety cabinets bsc conduct all procedures involving the manipulation of potentially infectious materials including aerosol producing activities when using aerosol producing equipment or when using high concentrations large volumes of organisms inside a biological safety cabinet. The air mixes with the recirculating air stream and passes through a hepa filter downward toward the work surface creating a contamination free zone.